Journal: British Journal of Cancer

Article Title: Insulin-like growth factor binding protein-6 inhibits prostate cancer cell proliferation: implication for anticancer effect of diethylstilbestrol in hormone refractory prostate cancer

doi: 10.1038/sj.bjc.6602520

Figure Lengend Snippet: Inhibition of the proliferation of human prostate cancer cell lines LNCaP and PC-3 by diethylstilbestrol (DES). Cells were incubated for 24 h, and thereafter CM was aspirated away and the cells were incubated with CM containing various concentrations of DES. After 72 h, the number of viable cells was measured by MTT assay. Optical densities (OD) of cell lysates were measured at a wavelength of 540 nm. The values are expressed as means+s.d. ( n =3), and the P -values were <0.05 ( * ) and <0.01 (†).

Article Snippet: The human prostate cancer cell lines LNCaP and PC-3 were purchased from Dainippon Pharmaceutical (Tokyo, Japan) and cultured in RPMI (Sigma, St Louis, MO, USA) supplemented with 10% fetal calf serum (FCS) (Moregate, Bulimba, Australia).

Techniques: Inhibition, Incubation, MTT Assay

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Structure–activity relationship of C-17 ester derivatives of andrographolide in the A549 cell line.

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: Activity Assay

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Effect of compound 9s at various doses on the A549 cell line’s nuclear morphology when stained with DAPI. Cisplatin (17 μM) was used as a positive control.

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: Staining, Positive Control

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Effect of compound 9s with different concentrations over ROS generation in the A549 cell line using DCFDA dye. Cisplatin was taken as positive control (17 μM).

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: Positive Control

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Mitochondrial membrane potential was measured using Rh-123 dye on treatment with different concentrations of 9s in A549 cells. Cisplatin was used as positive control (17 μM).

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: Membrane, Positive Control

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Cytotoxic Activity (% Growth Inhibition) for Ester Derivatives of Andrographolide at 20 μM Concentration

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: Activity Assay, Inhibition, Concentration Assay

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Results of Screening Using the MTT Assay a

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: MTT Assay

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Expanded Panel of Lung Cancer Cell Lines with Respect to Selectivity Index with the Normal Cell Line

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: